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Product Information
EcoRI has a High Fidelity version EcoRI-HF® (NEB #R3101).
High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
For details on NEB’s quality controls for restriction endonucleases, visit our Restriction Enzyme Quality page.Product Source
An E. coli strain that carries the cloned EcoRI gene from E. coli RY13 (R.N. Yoshimori).- This product is related to the following categories:
- Restriction Endonucleases C G Products,
- Time-Saver Qualified Restriction Enzymes Products
- This product can be used in the following applications:
- Fast Cloning: Accelerate your cloning workflows with reagents from NEB,
- Restriction Enzyme Digestion
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Protocols, Manuals & Usage
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Tools & Resources
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FAQs & Troubleshooting
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Citations & Technical Literature
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Product Citation Tool
Additional Citations
- Tong C, Li H, Wang Y, Li X, Ou J, Wang D, Xu H, Ma C, Lang X, Liu G, Zhang B, Shi J. (2016) Construction of High-Density Linkage Maps of Populus deltoides × P. simonii Using Restriction-Site Associated DNA Sequencing. PLoS One; Mar 10;11(3), e0150692.
- Yang H, Wei CL, Liu HW, Wu JL, Li ZG, Zhang L, Jian JB, Li YY, Tai YL, Zhang J, Zhang ZZ, Jiang CJ, Xia T, Wan XC. (2016) Genetic Divergence between Camellia sinensis and Its Wild Relatives Revealed via Genome-Wide SNPs from RAD Sequencing. PLoS One; Mar 10;11(3), e0151424.
- Mousavi M, Tong C, Liu F, Tao S, Wu J, Li H, Shi J. (2016) De novo SNP discovery and genetic linkage mapping in poplar using restriction site associated DNA and whole-genome sequencing technologies. BMC Genomics; Aug 18;17, 656.
- Xiao B, Tan Y, Long N, Chen X, Tong Z, Dong Y, Li Y. (2015) SNP-based genetic linkage map of tobacco (Nicotiana tabacum L.) using next-generation RAD sequencing. J Biol Res (Thessalon); Oct 6;22:11. ,
- Zhang Q, Liu C, Liu Y, VanBuren R, Yao X, Zhong C, Huang H. (2015) High-density interspecific genetic maps of kiwifruit and the identification of sex-specific markers. DNA Res; Oct;22(5), 367-75.
- Bonatelli IA, Carstens BC, Moraes EM. (2015) Using Next Generation RAD Sequencing to Isolate Multispecies Microsatellites for Pilosocereus (Cactaceae). PLoS One; Nov 11;10(11), e0142602.
- Fischer G, Azorsa F, Garcia FH, Mikheyev AS, Economo EP. (2015) Two new phragmotic ant species from Africa: morphology and next-generation sequencing solve a caste association problem in the genus Carebara Westwood. Zookeys; Oct 5;(525), 77-105.
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Featured Videos
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Reduce Star Activity with High-Fidelity Restriction Enzymes
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TIME-SAVER™ Protocol for Restriction Enzyme Digests
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NEB® TV Ep. 15 – Applications of Restriction Enzymes
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Restriction Enzyme Digest Protocol: Cutting Close to DNA End
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Restriction Enzyme Digestion Problem: DNA Smear on Agarose Gel
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Why is My Restriction Enzyme Not Cutting DNA?
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Restriction Enzyme Digest Problem: Too Many DNA Bands
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Double Digestion with NEBcloner
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