HindIII has a High Fidelity version HindIII-HF™ (NEB #R3104).
High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
HindIII crystals (Ira Schildkraut and Lydia Dorner, New England Biolabs)
Product Source
An E. coli strain that carries the HindIII gene from Haemophilus influenzae Rd (ATCC 51907).
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